OP05 Proteomic footprint of murine tumor-associated myeloid populations for myeloid cell reprogramming in cancer
نویسندگان
چکیده
Tumor microenvironment (TME) remodeling is one of the major research subjects in oncology. Several strategies can be implemented to modulate tumor microenvironment, particularly reprogramming myeloid cells stimulate their anti-cancer activities. Indeed, constitute component TME. Hence, it important identify molecular signatures associated cancer- promoting cells. Here, we defined phenotype and proteome Moreover, identified relationships between myeloid-derived suppressor (MDSCs) macrophages (TAM). The proteomic atlas tumor-associated revealed routes reprogram cancer-associated We used an ex vivo differentiation system for MDSCs TAM by from C57BL/6J mouse bone marrow cancer-polarized conditioning medium. also differentiated resting (M0) as controls using standard techniques. Flow cytometry microscopy confirmed assessing morphology presence characteristic lineage markers. Three global experiments quantitative mass spectrometry (shotgun proteomics) were performed. Construction functional interactomes maps up- or down-regulated proteins was conducted with Ingenuity Pathway Analysis (IPA) Tool Quiagen. evaluated effect at differentiation, maturation immunosuppressive level TAMs several compounds. Markers western blot. morphological phenotypic differences populations. High-throughput proteomics uncovered protein expression patterns populations modelling tumor-infiltrating subsets, a result cancer-derived factors. Therefore, compounds A resemblance activated greater rise DC observed. functions decreased which led enhanced CD4 proliferation increased ability release more IFN-gamma IL2. In present study, M-MDSCs, G-MDSC related lineage, cancer-driven polarization. these permit us develop cancer associated.
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ژورنال
عنوان ژورنال: ESMO open
سال: 2022
ISSN: ['2059-7029']
DOI: https://doi.org/10.1016/j.esmoop.2022.100681